In vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes.
نویسندگان
چکیده
Thylakoid membranes isolated from the cyanobacterium Synechocystis sp. strain PCC6803 were capable of desaturating the acyl groups in monogalactosyl diacylglycerol. This desaturation reaction required the reduced form of ferredoxin.
منابع مشابه
Comparative Analysis of Fatty Acid Desaturases in Cyanobacterial Genomes
Fatty acid desaturases are enzymes that introduce double bonds into the hydrocarbon chains of fatty acids. The fatty acid desaturases from 37 cyanobacterial genomes were identified and classified based upon their conserved histidine-rich motifs and phylogenetic analysis, which help to determine the amounts and distributions of desaturases in cyanobacterial species. The filamentous or N(2)-fixin...
متن کاملEffect of frost hardening on lipid and Fatty Acid composition of chloroplast thylakoid membranes in two wheat varieties of contrasting hardiness.
Lipid and fatty acid composition of chloroplast thylakoid membranes was determined in two varieties of wheat (Triticum aestivum L.), the hardy Miranovskaja and the sensitive Penjamo. Plants were grown at room temperature or under frost hardening conditions (1.5 degrees C). Changes in lipid and fatty acid composition of the isolated thylakoids could be related to the temperature dependence of li...
متن کاملCritical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism.
Photosynthetic microorganisms typically have multiple isoforms of the electron transfer protein ferredoxin, although we know little about their exact functions. Surprisingly, a Chlamydomonas reinhardtii mutant null for the ferredoxin-5 gene (FDX5) completely ceased growth in the dark, with both photosynthetic and respiratory functions severely compromised; growth in the light was unaffected. Th...
متن کاملInvolvement of ferredoxin in desaturation of lipid-bound oleate in chloroplasts.
Intact spinach (Spinacia oleracea) chloroplasts, pulse-labeled with [(14)C]acetate, desaturate newly formed fatty acids as ester groups of monogalactosyl diacylglycerol in a subsequent chase in the dark. Rupture of pulse-labeled chloroplasts by addition of a detergent solution 3-([3-cholamidopropyl]dimethylammonio)-1-propane sulfonate preserves part of this desaturation activity. Direct additio...
متن کاملDirect desaturation of intact galactolipids by a desaturase solubilized from spinach (Spinacia oleracea) chloroplast envelopes.
In plants, polyenoic fatty acids are synthesized by desaturase enzymes which use acyl groups of membrane lipids as substrates. To provide direct 'in vitro' evidence for this reaction, we solubilized envelope membranes from spinach (Spinacia oleracea) chloroplasts with Triton X-100 to release a membrane-bound n-6 desaturase. In the presence of oxygen and reduced ferredoxin, the solubilized enzym...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 175 2 شماره
صفحات -
تاریخ انتشار 1993